Project goal is to develop effective fed-batch fermentation and scale-up techniques for the production of plant-based meat substitutes, utilizing a recombinant LegH (leghemoglobine) producing strain of Pichia pastoris and Kluyveromyces lactis.

Main activities of the project:

The main activities of the project include the creation of two genetically modified yeast strains capable of utilizing cheap and freely available substrates for hemoprotein biosynthesis. As well as to develop the following: efficient fermentation technologies for both previously obtained yeast strains; mathematical models of processes; efficient model-based control systems that would allow automatic control of hemeprotein biosynthesis processes in both batch and fed-batch mode. In addition, the project plans to perform theoretical and practical scaling of hemeprotein production processes to a pilot scale, as well as to evaluate the commercialization potential of the developed fermentation process and related systems.

Project duration: 23 months.

Total eligible costs of the Project account for 540 540 EUR, including funding from the European Regional Development Fund in amount of 81.96 % – 443 026.58 EUR.